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目的了解在接近人类饮茶习惯状态下,低超常氟含量红碎茶对牙齿釉质形态结构、化学元素含量的影响。方法对饮用氟浓度为8.2mg/L和16.4mg/L红碎茶水360天后的144只大鼠牙齿的牙釉质,使用偏光显微镜、扫描电子显微镜、X线荧光光谱仪观察牙釉质形态结构,检测釉质中的化学元素含量。结果两种氟含量的红碎茶水均导致动物氟斑牙釉质出现过度钙化和钙化不全,呈现出剂量-效应关系。检测到动物氟斑牙釉质中含有钙、磷、镁、铝等12种化学元素。两个实验组牙齿釉质的钙、磷、镁含量显著下降,铝、氯含量显著上升。饮氟含量8.2mg/L红碎茶水组硅、硫、铁含量低于对照组,而在氟含量16.4mg/L红碎茶水组,这3种元素含量显著高于8.2mg/L组和对照组。结论长期饮用低超常量氟含量红碎茶水可导致机体慢性氟中毒,低超常氟含量红碎茶对釉质形态结构的损害主要表现为过度钙化和钙化不全同时存在。釉质中钙、磷含量下降,铝、氯含量增加,以及其他几种化学元素含量的改变提示,这些化学元素可能参与了影响釉质的发育、矿化过程。
OBJECTIVE: To investigate the effect of low ultra-normal fluorine content of broken tea on enamel morphological structure and chemical element content in tea drinking habits. Methods Tooth enamel of 144 rats after drinking for 360 days with fluoride concentration of 8.2mg/L and 16.4mg/L was used to observe enamel morphological structure and detect enamel using polarized light microscope, scanning electron microscope and X-ray fluorescence spectrometer. The chemical element content in the. RESULTS: Both of the two fluoride content of red broken tea resulted in the over-calcification and calcification of enamel dental enamel in animals, showing a dose-effect relationship. The enamel of animal fluorosis contained 12 chemical elements such as calcium, phosphorus, magnesium and aluminum. In the two experimental groups, the content of calcium, phosphorus and magnesium in tooth enamel decreased significantly, and the contents of aluminum and chlorine increased significantly. The content of silicon, sulfur, and iron in drinking water containing 8.2mg/L of red broken tea was lower than that in the control group, while the content of these three elements was significantly higher in 8.2mg/L of broken tea water than in the 8.2mg/L group and control. group. Conclusion Long-term consumption of low-constant-calorie red fluoride-containing tea water can lead to chronic fluorosis in the body, and damage to enamel morphological structure of red-crushed tea with low-ultra-normal fluorine content is mainly due to the presence of excessive calcification and calcification insufficiency. The decrease of calcium and phosphorus content in enamel, the increase of aluminum and chlorine content, and the changes in the content of several other chemical elements suggest that these chemical elements may be involved in the development and mineralization of enamel.