【摘 要】
:
通过人工隔离诱导实现新疆早实核桃的孤雌生殖,孤雌生殖频率达8.17%~69.44%,且播种后孤雌生殖核桃出苗率可达5.26%~80%.研究过程中发现的品种核桃天然孤雌生殖植株,通过人工套
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通过人工隔离诱导实现新疆早实核桃的孤雌生殖,孤雌生殖频率达8.17%~69.44%,且播种后孤雌生殖核桃出苗率可达5.26%~80%.研究过程中发现的品种核桃天然孤雌生殖植株,通过人工套袋隔离促其孤雌生殖,获得2株生长正常的孤雌生殖核桃播种苗.采用RAPD技术对孤雌品种进行鉴定,共用55条引物对其4个材料(914母本、914孤雌、914单株-1、914单株-2)进行扩增,结果表明这四个材料均无差异带出现,说明所有供试材料在基因组的大约450多个位点扩增的DNA片段没有差异,证明914孤雌单株与其母本无显著差异、为纯化基因型植株.利用孤雌生殖开展核桃优良品种遗传纯化,可最大限度地缩短纯化周期,为林木遗传纯化开拓一条快捷、高效、实用的新途径.
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