应用改进寇氏法，测定复方硫酸氢黄连素灌肠液肛注腹腔注射等不同给药途径半数致死量（LD50），并选用大鼠、家兔及豚鼠，对其局部刺激性及过敏性进行观察。结果，其LD50值分别为228.93mg／kg和15.88mg／kg。对大鼠直肠粘膜无毒性，对家兔股四头肌有轻度刺激性，对豚鼠致敬反应阴性。可见，复方硫酸氢黄连素灌肠液是一种安全的新型制剂. The improved method was used to determine the median lethal dose (LD50) of various injection routes such as intraperitoneal injection of compound rhubarbitin sulfate enema, and rats, rabbits and guinea pigs were used to observe the local irritation and anaphylaxis. . As a result, the LD50 values ​​were 228.93 mg/kg and 15.88 mg/kg, respectively. It has no toxicity to rat rectal mucosa, mild irritation to quadriceps of rabbits, and negative reaction to guinea pigs. 5. It can be seen that the compound sulphate hydrogen sulphate enema is a safe new agent.