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[Objective] To determine the content of scutellarin in Erigeron breviscapus(Vant.) Hand Mazz.from different habitats of Yunnan Province.[Method] The content of scutellarin was determined by HPLC,and the HPLC conditions were as follows:Shim-pack VP-ODS column(150.0 mm×4.6 mm,5 μm),mobile phase of methanol-0.1% phosphoric acid(V/V,40:60),flow rate of 1.0 ml/min,column temperature of 25 ℃ and detective wavelength of 335 nm.[Result] Erigeron breviscapus(Vant.) Hand Mazz.from 8 habitats of Yunnan Province differed from each other in scutellarin.Scutellarin in Erigeron breviscapus from Zhaotong Drug Hill was the highest(2.628%),while that from Shuanglong Town of Kunming was the lowest(1.597%).[Conclusion]With good precision and repeatability,the method established in this case is suitable for the determination of scutellarin in Erigeron breviscapus,and this study provides basis for the breeding of improved variety and GAP cultivation of Erigeron breviscapus(Vant.) Hand Mazz..
[Objective] To determine the content of scutellarin in Erigeron breviscapus (Vant.) Hand Mazz. From different habitats of Yunnan Province. [Method] The content of scutellarin was determined by HPLC, and the HPLC conditions were as follows: Shim-pack VP Mobile phase of methanol-0.1% phosphoric acid (V / V, 40:60), flow rate of 1.0 ml / min, column temperature of 25 ° C and detective wavelength of -ODS column (150.0 mm × 4.6 mm, 335 nm. [Result] Erigeron breviscapus (Vant.) Hand Mazz. From 8 habitats of Yunnan Province differed from each other in scutellarin. Cucumberlarin in Erigeron breviscapus from Zhaotong Drug Hill was the highest (2.628%), while that from Shuanglong Town of Kunming was the lowest (1.597%). [Conclusion] With good precision and repeatability, the method established in this case is suitable for the determination of scutellarin in Erigeron breviscapus, and this study provides basis for the breeding of improved variety and GAP cultivation of Erigeron breviscapus (Vant.) Hand Mazz ..