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目的：观察急性白血病（ＡＬ）与中枢神经系统白血病（ＣＮＳ—Ｌ）患者血清与脑脊液（ＣＳＦ）中肿瘤坏死因子α（ＴＮＦα）水平变化，并探讨其临床意义。方法：用酶联免疫检测盒，采用双抗体夹心方法（ＥＬＩＳＡ），同时检测３６例ＡＬ患者与１３例正常对照组血清与ＣＳＦ中ＴＮＦα水平。结果：各型急性白血病患者血清与ＣＳＦ中ＴＮＦα均明显升高，并发ＣＮＳ—Ｌ后ＣＳＦ中ＴＮＦα进一步升高；急性淋巴细胞白血病（ＡＬＬ）患者血清ＴＮＦα与骨髓内白血病细胞百分数呈正相关；经治疗ＡＬ及ＣＮＳ—Ｌ皆完全缓解（ＣＲ）后血清与ＣＳＦ中ＴＮＦα明显下降，但仍不同程度高于正常。结论：检测各型ＡＬ及ＣＮＳ—Ｌ患者血清与ＣＳＦ中ＴＮＦα水平对于其诊断、治疗转归皆有重要意义。
Objective: To observe the changes of tumor necrosis factor α (TNFα) levels in serum and cerebrospinal fluid (CSF) in patients with acute leukemia (AL) and central nervous system leukemia (CNS-L), and to explore its clinical significance. METHODS: TNFα levels in serum and CSF of 36 AL patients and 13 normal controls were detected by enzyme-linked immunosorbent assay (ELISA). Results: The levels of TNFα in serum and CSF of all types of acute leukemia patients were significantly increased, and TNFα in CSF increased further after CNS-L. The serum TNFα in patients with acute lymphoblastic leukemia (ALL) was positively correlated with the percentage of leukemia cells in bone marrow; Both AL and CNS-L were completely relieved (CR) and serum TNFα in CSF was significantly decreased, but it was still higher than normal. Conclusion: The detection of TNFα levels in serum and CSF in patients with various types of AL and CNS-L has important significance in the diagnosis and treatment outcome.