文章简介长链非编码RNA(lncRNA)是一类长度大于200nt且不编码蛋白质的RNA转录物。近年来,随着高通量测序的普及越来越多的lncRNA被鉴定出来,据估算人和小鼠的基因组中lncRNA基因和蛋白编码基因数量基本相当。但关于lncRNA的功能研究一直存在争议。到目前为止,只有少数lncRNA被证明是有功能的。沈晓骅实验室创新性地利用CRISPR/Cas9系统介导的敲除、敲入等策略,系统全面地研究了一个长链非编 Introduction Short chain non-coding RNAs (lncRNAs) are a class of RNA transcripts that are greater than 200 nt in length and do not encode a protein. In recent years, with the increasing popularity of high-throughput sequencing more and more lncRNAs are identified, it is estimated that the number of lncRNA genes and protein-coding genes in the human and mouse genomes is roughly equivalent. However, the research on the function of lncRNA has been controversial. To date, only a few lncRNAs have been shown to be functional. Shen Xiaohua laboratory innovative use of CRISPR / Cas9 system-mediated knocking, knocking and other strategies, a comprehensive study of a long chain non-coding