论文部分内容阅读
目的 检测分析己酮可可碱抗小鼠血吸虫病肝纤维化前后血清TNF -α ,IFN -γ水平的变化。方法 建立血吸虫病肝纤维化小鼠模型 ,应用ELISA方法检测血吸虫病肝纤维化小鼠在己酮可可碱治疗前后血清TNF -α ,IFN -γ水平的变化。结果 己酮可可碱治疗可降低血清TNF -α的含量 ,高剂量治疗组与感染组相比P <0 0 1,而低剂量组与感染组相比P<0 0 5 ,高剂量组与低剂量组间有极显著性差异 (P <0 0 1) ;己酮可可碱治疗后可提高IFN -γ的含量 ,高剂量己酮可可碱治疗组和低剂量治疗组与感染组相比P值均小于 0 0 1,高剂量组与低剂量组间有显著性差异 (P <0 0 5 ) ;高剂量治疗组疗效与吡喹酮治疗组相比无统计学上的差异 (P >0 0 5 )。结论 己酮可可碱可明显降低血吸虫病肝纤维化小鼠血清TNF -α ,提高IFN -γ的水平从而发挥其抗肝纤维化的作用 ,作用与剂量呈正相关
Objective To detect the changes of serum levels of TNF-α and IFN-γ before and after pentoxifylline against mouse schistosomiasis with liver fibrosis. Methods The mouse model of schistosomiasis hepatic fibrosis was established. The serum levels of TNF-α and IFN-γ in schistosomal liver fibrosis mice before and after pentoxifylline treatment were detected by ELISA. Results Pentoxifylline could decrease the level of serum TNF-α, P <0 01 in high-dose treatment group and P 0 05 in low-dose treatment group and high-dose treatment group There was a significant difference between the dosage groups (P <0.01); pentoxifylline increased the content of IFN-γ, the pentoxifylline treatment group and the low-dose treatment group compared with the infection group (P <0 05). There was no significant difference between high-dose treatment group and praziquantel treatment group (P> 0 0) 5). Conclusion Pentoxifylline can significantly reduce the serum levels of TNF-α and enhance the level of IFN-γ in schistosomiasis mice with hepatic fibrosis and thus exert its anti-hepatic fibrosis effect, which is positively correlated with the dosage