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目的探讨趋化因子受体CXCR7在胰腺癌发生发展中的作用。方法应用shRNA构建稳定敲除CXCR7的胰腺癌细胞,并采用MTT法、流式细胞术和侵袭实验分别检测胰腺癌细胞的增殖、细胞周期、凋亡和侵袭能力变化。结果与空白对照组相比,转染CXCR7-shRNA慢病毒表达载体AsPC-1细胞中CXCR7在mRNA及蛋白水平表达均显著降低(P<0.05),细胞增殖和侵袭能力显著降低(P<0.05),胰腺癌细胞休眠于G0/G1期,同时细胞凋亡率明显增加。结论CXCR7在调节胰腺癌细胞生长和侵袭能力中扮演重要角色,并且为胰腺癌的治疗提供了可能的潜在靶点。
Objective To investigate the role of chemokine receptor CXCR7 in the development of pancreatic cancer. Methods Pancreatic cancer cells stably knocked out CXCR7 were constructed by shRNA. The proliferation, cell cycle, apoptosis and invasiveness of pancreatic cancer cells were detected by MTT assay, flow cytometry and invasion assay respectively. Results Compared with the blank control group, the expression of CXCR7 mRNA and protein in AsPC-1 cells transfected with CXCR7-shRNA lentivirus was significantly decreased (P <0.05), and the proliferation and invasion ability of CXCR7-shRNA lentivirus was significantly decreased (P <0.05) , Pancreatic cancer cells dormancy in G0 / G1 phase, at the same time apoptosis rate increased significantly. Conclusion CXCR7 plays an important role in the regulation of pancreatic cancer cell growth and invasion and provides a potential target for the treatment of pancreatic cancer.