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目的通过测定婴幼儿喘息患儿鼻咽吸出物中细胞因子IL-4、IL-12和IFN-γ的水平变化,探讨婴幼儿喘息与机体细胞因子反应类型的关系。方法应用固相夹心ELISA法测定和比较58例婴幼儿喘息和47例无喘息的婴幼儿肺炎鼻咽吸出物IL-4、IL-12和IFN-γ水平变化,同时对急性期和恢复期婴幼儿喘息鼻咽吸出物IL-4、IL-12和IFN-γ水平进行测定和比较。结果与婴幼儿肺炎组[(275.11±30.27)ng/L]、对照组[(253.76±39.9)ng/L]和婴幼儿喘息恢复期组[(260.88±28.07)ng/L]比较,鼻咽吸出物IFN-γ水平在婴幼儿喘息组急性期[(142.55±26.56)ng/L]明显下降(P<0.01),而IL-4水平[分别为(8.51±2.02)ng/L,(3.14±1.16)ng/L,(4.95±1.93)ng/L,(3.28±1.36)ng/L)]和IL-4/IFN-γ(分别为0.068±0.044,0.012±0.004,0.019±0.007,0.014±0.005)比值则明显升高(P<0.05);IL-12在婴幼儿喘息急性期组[(587.24±58.14)ng/L]明显低于肺炎组[(1 322.13±139.74)ng/L](P<0.01),与恢复期组[(580.77±64.32)]ng/L和对照组[(604.02±88.38)ng/L]比较差异无统计学意义(P>0.05)。结论婴幼儿喘息急性期局部主要表现为Th2型细胞免疫反应,鼻咽吸出物IL-4产生明显增加,IFN-γ水平则明显下降,而IL-12在婴幼儿肺炎中产生增加。
Objective To investigate the relationship between the wheezing of infants and young children and the type of cytokine response in infants and young children by measuring the changes of cytokines IL-4, IL-12 and IFN-γ in nasopharyngeal aspirates from children with asthma. Methods The levels of IL-4, IL-12 and IFN-γ in nasopharyngeal aspirates from 58 infant infants and 47 infants without pneumonia were measured and compared by solid-phase sandwich ELISA. The acute and convalescent infants Children asthma nasopharyngeal aspirates IL-4, IL-12 and IFN-γ levels were measured and compared. Results Compared with infants and young children pneumonia group (275.11 ± 30.27 ng / L), control group (253.76 ± 39.9 ng / L) and infants wheezy recovery group (260.88 ± 28.07 ng / L), nasopharyngeal The level of IFN-γin superoxide group was significantly lower than that in the asthmatic group [(142.55 ± 26.56) ng / L] and IL-4 level [8.51 ± 2.02ng / ± 1.16 ng / L, 4.95 ± 1.93 ng / L, 3.28 ± 1.36 ng / L and IL-4 / IFN- ± 0.005) (P <0.05). The IL-12 level in asthmatic children [(587.24 ± 58.14) ng / L] was significantly lower than that in pneumonia group [(13232 ± 139.74) ng / L] (P <0.01). There was no significant difference between the recovery group [(580.77 ± 64.32) ng / L and the control group (604.02 ± 88.38) ng / L] (P> 0.05). Conclusion Th1 type immune response mainly occurs in the acute stage of infantile wheezing. The IL-4 production in nasopharyngeal aspirates is obviously increased, the level of IFN-γ is significantly decreased, while IL-12 is increased in infantile pneumonia.