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长期骨髓培养(LTBMC)可用来研究骨髓细胞的增殖能力。本文研究25例(男15,女10)未经治疗的MDS患者(其中RA7例,RSA和RAEB各8例,RAEB-t和CMML各1例)和30例(男20,女10)正常骨髓的LTBMC,培养后进行集落分析,LTBMC结果与常规的短期克隆测定相对比。结果:在最初骨髓体外培养中,与正常对比,25例MDS患者祖细胞分布比较分散,中数值较低,为50％。56％的培养中,克隆很少或完全缺如。有些仅有集落或克隆为主,这些异常生长,RA/RSA占53％,RAEB/RAEB-t占44％。这与骨髓中原始细胞百分率无关。1例CMML患者骨髓可见高克隆形成。在LTBMC的细胞增殖和分化中,19例患者(RA
Long-term bone marrow culture (LTBMC) can be used to study the proliferation of bone marrow cells. In this study, we studied 25 untreated MDS patients (7 RA7, 8 RSA and RAEB, 1 RAEB-t and 1 CMM) and 30 normal (20 and 10 female) MDS patients Of LTBMCs were cultured and then subjected to colony analysis. The results of LTBMC were compared with those of conventional short-term cloning assays. RESULTS: In the initial bone marrow in vitro culture, progenitor cells were more dispersed in 25 patients with MDS compared with normal, with a lower median of 50%. In 56% of the cultures, there are few or no clones. Some were only colonies or clones. These abnormalities grew with 53% of RA / RSA and 44% of RAEB / RAEB-t. This is not related to the percentage of blasts in the bone marrow. One case of CMML patients showed high clone formation in bone marrow. In LTBMC cell proliferation and differentiation, 19 patients (RA