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目的探讨具有通络作用的单味代表药的通络效果,并将其与复方通心络的通络作用进行比较。方法建立内皮损伤、内皮损伤合并微血栓形成(络脉瘀阻)及内皮损伤合并急性心肌缺血(络脉绌急)大鼠模型,分别检测血管舒张功能、血浆NO和血管性假血友病因子(v WF)水平;凝血功能和血小板计数(PLT),镜下观测肠系膜微循环,监测心电图T波改变。结果内皮损伤模型中,各用药组最大舒张率、血浆NO值均显著高于模型组,其中通心络组升高更为明显;各用药组血浆v WF值显著低于模型组,其中通络组降低更为明显。在络脉瘀阻模型中,各用药组肠系膜微循环血液流态较模型组均有明显改善;各用药组的纤维蛋白原(FG)水平与模型组比较显著升高;通心络组和水蛭组血浆v WF值显著低于模型组,PLT显著高于模型组。在络脉绌急模型中,通心络组在30 s~3 min各时间点T波压低幅度与模型组相比均显著降低;蜈蚣组在注射精氨酸加压素(AVP)后30 s~2min时T波压低幅度较模型组明显减少;各用药组血浆NO值均显著高于模型组,但通心络组升高更为明显。结论通络单味代表药人参、薤白、蜈蚣、水蛭对血管内皮损伤、络脉瘀阻以及络脉绌急有一定的防治作用,但其作用弱于复方通心络。通心络的通络效应优于各单一的通络药物,证明组方的科学性。
Objective To investigate the effect of meridian-collaterals with single-agent on meridian and to compare its meridian effect with compound tongxinluo. Methods Endothelial injury, endothelial injury with microthrombosis (collaterals vein stasis) and endothelial injury with acute myocardial ischemia (collaterals urgency) rat model were established, and the effects of vasodilation, plasma nitric oxide and von Willebrand disease Factor (v WF) level, coagulation function and platelet count (PLT). The mesenteric microcirculation was observed microscopically and T wave changes were monitored. Results In the model of endothelial injury, the maximal diastolic rate and plasma NO in each drug group were significantly higher than those in the model group, especially in Tongxinluo group; the plasma vWF values in each drug group were significantly lower than those in the model group Group decreased more obvious. In the model of collaterals and stasis, the mesenteric microcirculation blood flow of each treatment group was significantly improved compared with the model group; the fibrinogen (FG) level of each treatment group was significantly increased compared with the model group; Tongxinluo group and Leech Plasma vWF was significantly lower in model group than in model group, and PLT was significantly higher than model group. In the acute collateral deficit model, the amplitude of T wave depression in Tongxinluo group was significantly lower than that in model group at each time point ranging from 30 s to 3 min, while in centipede group, 30 s after AVP The amplitude of T wave decreased significantly at ~ 2min compared with the model group. The plasma NO level of each drug group was significantly higher than that of model group, but the increase of Tongxinluo group was more obvious. Conclusions Tongluo, a typical herb, has certain preventive and therapeutic effects on the injury of vascular endothelium, collaterals, collaterals and collaterals, but its effect is weaker than compound Tongxinluo. Tongxinluo meridian effect is better than the single drug Tongluo to prove the scientific side of the group.