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本文报告用PCR的体外基因扩增技术对116例弓形虫(TOX)抗体(IgM/IgG)检测阳性产妇的新生儿脐带血作PCR检查,结果PCR阳性29例,阳性率为25%;同时用ELISA法检测脐血中弓形虫特异性抗体IgG和IgM,阳性率分别为27.6%和16.3%,提示应用PCR技术检测脐血中弓形虫DNA结合ELISA进行筛选,可早期诊断新生儿隐匿型先天性弓形虫病,以利于及时治疗,减少或减轻隐匿型先天性弓形虫病的后发症,降低其危害。
In this study, PCR-based in vitro gene amplification was used to detect the cord blood of 116 newborns with positive mothers who had tested for the presence of TOX antibody (IgM / IgG). PCR was performed in 29 of them, with a positive rate of 25% ELISA method for the detection of Toxoplasma gondii specific IgG and IgM in cord blood was 27.6% and 16.3% respectively, suggesting that PCR-based detection of Toxoplasma gondii DNA-binding ELISA in cord blood could be used for early diagnosis of occult neonate Congenital toxoplasmosis, in order to facilitate the timely treatment, reduce or reduce the incidence of occult congenital toxoplasmosis, reduce its harm.