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研究目的:2006年我国上海首次发现番茄黄化曲叶病毒(TYLCV),随后TYLCV迅速蔓延至全国13个省份和自治区。本研究分析了2006至2010年期间TYLCV在我国首发地上海市的分子变异规律。创新要点:本研究持续五年追踪田间TYLCV,分析TYLCV的全长基因组序列、分子变异及种群遗传结构,为防控TYLCV提供理论依据。研究方法:2006至2010年从上海采集了26个TYLCV的分离物,利用高保真性的滚环扩增技术获得TYLCV分离物的全长基因组。应用MEGA5等生物信息学软件分析TYLCV的分子变异。重要结论:TYLCV自然种群具有与RNA病毒相似的突变率,以基因间隔区的分子变异最大,平均突变率为4.81×10-3。TYLCV的大部分基因都处于负向选择,但包含在C1开放阅读框内的C4,却与C1承受着不同的选择压而处于正向选择。
Research purposes: In 2006, Shanghai first discovered tomato yellow leaf curl virus (TYLCV), TYLCV then rapidly spread to 13 provinces and autonomous regions. This study analyzed the molecular variation of TYLCV in Shanghai from 2006 to 2010. Innovative Points: In this study, TYLCV was traced in the field for five years and the full-length genomic sequence, molecular variation and population genetic structure of TYLCV were analyzed to provide a theoretical basis for the prevention and control of TYLCV. METHODS: From 2006 to 2010, 26 TYLCV isolates were collected from Shanghai and the full-length genome of TYLCV isolates was obtained using high-fidelity rolling circle amplification. The molecular variation of TYLCV was analyzed by bioinformatics software such as MEGA5. Important conclusion: The natural population of TYLCV has the same mutation rate as the RNA virus, with the largest molecular variation in the intergenic region with an average mutation rate of 4.81 × 10-3. Most of the TYLCV genes are in negative selection, but C4 contained in the C1 open reading frame is positively selected with a different selection pressure on C1.