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为探讨蜂胶黄酮Pinobanksin-3-acetate(PB3A)对人肝癌Hep G-2和肝正常细胞L02增殖、形态学变化和细胞凋亡的影响,采用MTT显色法检测不同浓度PB3A作用不同时间对SGC-7901细胞生长所产生的影响,计算生长抑制率和IC50值;倒置显微镜观察PB3A干预后细胞的形态学变化;Annexin V-FITC/PI双染色、流式细胞仪检测细胞的凋亡率;结果显示,PB3A可显著抑制Hep G-2和L02细胞的增殖,并呈浓度和时间依赖性,各时间段Hep G-2细胞IC50值明显低于L02细胞。流式细胞检测结果显示,PB3A能促进Hep G-2和L02细胞凋亡,并呈剂量依赖性,同浓度PB3A诱导Hep G-2细胞的早期凋亡率明显高于L02细胞。上述结果表明,PB3A对人正常肝L02细胞和肝癌Hep G-2具有明显的区别增殖抑制和诱导凋亡作用,区别抑制作用的本质是PB3A诱导两种细胞凋亡的程度不同。
To investigate the effects of propolis flavone Pinobanksin-3-acetate (PB3A) on the proliferation, morphological changes and apoptosis of human hepatocellular carcinoma Hep G-2 and normal liver cells L02, MTT assay was used to detect the effects of different concentrations of PB3A on SGC -7901 cell growth, the growth inhibition rate and IC50 value were calculated. Morphological changes of cells were observed by inverted microscope after the intervention of PB3A; Annexin V-FITC / PI double staining and flow cytometry were used to detect the apoptosis rate; Results The results showed that PB3A significantly inhibited the proliferation of Hep G-2 and L02 cells in a concentration-and time-dependent manner. The IC50 of Hep G-2 cells in each time period was significantly lower than that in L02 cells. Flow cytometry showed that PB3A could promote the apoptosis of Hep G-2 and L02 cells in a dose-dependent manner. The early apoptosis rate of Hep G-2 cells induced by PB3A at the same concentration was significantly higher than that of L02 cells. The above results show that PB3A has a significant difference between human normal liver L02 cells and Hep G-2 hepatoma Hep G-2 proliferation inhibition and induction of apoptosis, the difference is PB3A-induced apoptosis in two different levels.