论文部分内容阅读
目的:采用RP-HPLC法测定并比较不同产地丹参中丹参酮ⅡA和丹酚酸B的含量。方法:采用高效液相色谱法,Agilent ZORBAX SB-C18(4.6mm×250mm,5μm)色谱柱,以甲醇-水(75∶25)为流动相,检测波长270nm测定丹参酮IIA;以甲醇-5%乙酸(35∶65)为流动相,检测波长288nm测定丹酚酸B。流速均为1mL/min,柱温均为25℃。结果:15批丹参药材中丹参酮ⅡA的含量为0.24%~0.47%;丹酚酸B的含量为3.16%~4.25%。结论:不同产地丹参中丹参酮ⅡA和丹酚酸B的含量存在一定差异。其中丹参酮IIA含量以10号样品(安徽太和)为最高,14号样品(江苏盐城)为最低;丹酚酸B含量以6号样品(河南焦作)为最高,1号样品(山东莒县)为最低。
Objective: To determine and compare the content of tanshinone ⅡA and salvianolic acid B in Radix Salviae Miltiorrhizae by RP-HPLC. Methods: High performance liquid chromatography (HPLC) was performed on an Agilent ZORBAX SB-C18 (4.6 mm × 250 mm, 5 μm) column using methanol-water (75:25) as mobile phase at a detection wavelength of 270 nm for the determination of tanshinone IIA. Acetic acid (35:65) as the mobile phase, the detection wavelength of 288nm determination of salvianolic acid B. Flow rates were 1mL / min, column temperature was 25 ℃. Results: The content of tanshinone ⅡA in 15 batches of Radix Salviae miltiorrhizae was 0.24% -0.47% and the content of salvianolic acid B was 3.16% -4.25%. Conclusion: The contents of tanshinone ⅡA and salvianolic acid B in Radix Salviae Miltiorrhizae from different areas have some differences. Among them, the content of tanshinone IIA was the highest in No. 10 sample (Taihe of Anhui Province), the lowest in No. 14 sample (Yancheng of Jiangsu); the content of salvianolic acid B was the highest in No. 6 sample (Jiaozuo of Henan) For the lowest.