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目的观察多功能钙/钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)在碘缺乏和甲状腺功能减退的大鼠仔鼠海马中蛋白表达的影响。方法健康2月龄雌性Wistar大鼠,交配妊娠后,取孕鼠28只,按体重随机分成对照组、甲状腺功能减退组和碘缺乏组,甲状腺功能减退组根据饮水中含丙基硫尿嘧啶(PTU)剂量分为5和15mg/L组,每组7只孕鼠。分别于出生后(PN)7、14、和21天时,每组随机取5只仔鼠,灌流固定大脑,进行组织病理切片和免疫组化染色,观察分析海马CaMKⅡ表达。结果对照组仔鼠海马CA1、CA3和DG区均呈强阳性染色,在神经元细胞胞浆内充满CaMKⅡ。5、15mg/L和碘缺乏组仔鼠海马免疫反应产物的分布与对照组一致,但与对照组相比染色强度逐渐降低。PN21和PN14时,仔鼠海马CA1、CA3和DG区平均积分光密度在碘缺乏组[PN21:(26.05±4.98)、(30.79±3.22)、(26.40±2.63);PN14:(25.48±4.87)、(44.17±5.91)、(26.41±3.01)]和15mg/L组[PN21:(17.02±2.68)、(24.57±6.62)、(20.18±4.05);PN14:(20.66±3.51)、(34.94±5.09)、(27.32±4.97)]明显低于对照组[PN21:(57.75±13.22)、(65.03±6.20)、(49.39±8.41),P<0.05;PN14:(54.08±12.00)、(71.04±5.07)、(78.52±12.42),P<0.05]。PN7时,碘缺乏组和15mg/L组仔鼠海马CA1区平均积分光密度在碘缺乏组(25.74±3.33)和15mg/L组(26.89±5.25)明显低于对照组(40.53±3.65),P<0.05。结论碘缺乏和甲状腺功能减退可下调海马组织CaMKⅡ蛋白表达。
Objective To investigate the effect of CaMK Ⅱ on protein expression in hippocampus of neonatal rats with iodine deficiency and hypothyroidism. Methods Healthy 2-month-old female Wistar rats were randomly divided into control group, hypothyroidism group and iodine deficiency group according to their body weight. The hypothyroidism group was treated with propylthiouracil PTU) doses were divided into 5 and 15mg / L group, each group of 7 pregnant rats. Five rats were randomly selected from each group at 7, 14 and 21 days after birth (PN). Peripheral blood was collected and fixed for histopathology and immunohistochemistry. The expression of CaMKⅡ in the hippocampus was observed and analyzed. Results The hippocampal CA1, CA3 and DG regions in the control group showed strong positive staining, and the cytoplasm of neurons were filled with CaMK Ⅱ. The distribution of immunoreaction products in hippocampus of 15, 15 mg / L and iodine deficiency groups was consistent with that of the control group, but the staining intensity decreased gradually compared with the control group. PN21 and PN14, the average integral optical density of CA1, CA3 and DG in the hippocampus of neonatal rats was significantly higher than that in the iodine deficiency group [PN21: (26.05 ± 4.98), (30.79 ± 3.22), (26.40 ± 2.63), PN14: (25.48 ± 4.87) , (14.17 ± 5.91), (26.41 ± 3.01) and 15mg / L group [PN21: (17.02 ± 2.68), (24.57 ± 6.62), (20.18 ± 4.05) (P <0.05); PN14: (54.08 ± 12.00), (71.04 ± 5.00), P <0.05), (27.32 ± 4.97) 5.07), (78.52 ± 12.42), P <0.05]. At PN7, the average integrated optical density of hippocampal CA1 region in iodine deficiency group and 15mg / L group was significantly lower than that in control group (25.74 ± 3.33) and 15mg / L group (26.89 ± 5.25) compared with control group (40.53 ± 3.65) P <0.05. Conclusion Iodine deficiency and hypothyroidism may down-regulate the expression of CaMKⅡ in hippocampus.