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目的:探讨二至丸对乙醇诱导的大鼠急性肝损伤的保护作用及其作用机制。方法:雄性大鼠72只随机分成6组,每组12只,分别为正常对照组,模型组,二至丸低、中、高剂量组(3.78,7.56,15.12 g·kg-1),阳性对照凯西莱60 mg·kg-1组。模型组以56%二锅头酒ig给药10 d,后开始给药,连续7 d,采用乙醇灌胃法建立大鼠急性乙醇性肝损伤模型,检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乙醇脱氢酶(ADH)、甘油三酯(TG)活性和肝匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)含量,观察肝脏病理形态学改变。结果:与模型组比较二至丸各剂量组均能显著降低血清ALT,AST,ADH水平;二至丸的高、中剂量组能显著降低肝匀浆中MDA含量,提高SOD活力;高剂量组可显著降低TG活性;通过病理学切片观察,二至丸各剂量组均能显著改善肝组织的病理变化,以二至丸高剂量效果最好。结论:二至丸对大鼠急性乙醇性肝损伤具有保护作用。
Objective: To investigate the protective effect of Erzhi Pill on alcohol-induced acute liver injury in rats and its mechanism. Methods: Seventy two male SD rats were randomly divided into 6 groups (n = 12): normal control group, model group and Erzhi low, middle and high dose groups (3.78, 7.56 and 15.12 g · kg -1) Control Casey 60 mg · kg-1 group. The model group was treated with 56% Erguotouji ig for 10 days and then began to be administered for 7 consecutive days. The model of acute alcoholic liver injury was established by ethanol gavage. Serum levels of alanine aminotransferase (ALT), aspartate AST, ADH, TG, SOD and MDA in liver homogenate were measured to observe the pathological changes of liver. Results: Compared with the model group, Erzhiwan dose group could significantly reduce the level of serum ALT, AST and ADH; Erzhiwan high and medium dose groups could significantly reduce the content of MDA in liver homogenate and increase the activity of SOD; Can significantly reduce the TG activity; observed by pathological sections, Erzhi pills each dose group can significantly improve the pathological changes of liver tissue, Erzhiwan high doses of the best. Conclusion: Erzhi Pills has a protective effect on acute alcoholic liver injury in rats.