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采用锯木屑培养担子菌,除非是在无菌条件下进行培养,不然几乎都会受到木霉菌等杂菌的污染。为了防止这种污染,通常采取如下两种措施:①先用常规方法对培养基进行灭菌处理,接上菌种后置5~10℃的低温下培养,能抑制杂菌的生长;②采用苯并咪唑衍生物等药剂,来抑制培养中的杂菌。然而,采用5~10℃的低温培养,会延长担子菌的培养时间;采用药物抑
Sawdust is used to culture Basidiomycetes unless it is cultivated aseptically, otherwise it is almost always contaminated by bacteria such as Trichoderma. In order to prevent this pollution, usually take the following two measures: ① first by conventional methods of medium sterilization, then strain on the set 5 ~ 10 ℃ low temperature culture, can inhibit the growth of bacteria; ② using Benzimidazole derivatives and other drugs, to inhibit the culture of bacteria. However, the use of low temperature culture 5 ~ 10 ℃, will extend the basidiomycete culture time; the use of drugs