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在筛选12种人工合成小分子肽影响大鼠离体黄体细胞孕酮生成的活性研究中,我们曾发现二肽甘氨酰—酪氨酰胺(Gly-Tyr-NH_2)和三肽甘氨酰—丝氨酰—赖氨酸(GlySer-Lys)有很强的抑制孕酮生成的作用。本文研究了这两种小肽的可能作用途径。实验观察到:蛋白质合成抑制剂放线菌酮(Cyx)和放线菌素D(ActD)对两种小肽拮抗孕酮分泌的作用没有影响;此两种小肽对孕酮合成的关键酶3 β-HSD活性也没有影响;但这两种小肽对腺苷酸环化酶激动剂Forskolin引起的孕酮分泌有抑制作用。提示这两种小肽可能抑制腺苷酸环化酶活性或影响cAMP以后的过程。
In the screening of 12 kinds of synthetic small molecule peptides affect progesterone production in rat luteal cells in vitro activity, we have found that dipeptide glycyl - tyrosin amide (Gly-Tyr-NH2) and tripeptide glycyl- Serine-lysine (GlySer-Lys) has a strong inhibitory effect on progesterone production. This article studies the possible ways of these two small peptides. It was observed experimentally that the protein synthesis inhibitors Cyx and ActD had no effect on antagonizing the secretion of progesterone by two small peptides; 3 β-HSD activity; however, these two small peptides have an inhibitory effect on progesterone secretion induced by adenosine cyclase agonist Forskolin. Suggesting that these two small peptides may inhibit adenylyl cyclase activity or affect the course of cAMP later.