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目的研究BALB/c小鼠免疫接种HBV表面S抗原疫苗(HBV small surface vaccine, S-HBsAg)能否引起特异性细胞毒T淋巴细胞(CTL)反应。方法分别给BALB/c小鼠腹腔内接种0~6 μg S-HBsAg,两周后加强一次,4周后分离小鼠脾细胞,体外用S-HBsAg特异性CTL表位多肽刺激;用特异性多肽、51Cr标记的P815细胞作为靶细胞;4 h51Cr释放实验检测CTL反应。结果分别接种0、0.65、1.25、2.5、5μg S-HBsAg的小鼠,其脾淋巴细胞CTL特异性释放率分别为31.21%±9.23%、42.36%±19.32%、91.21%±22.97%、69.25%±24.13%、51.49%±21.661%;只接受一次免疫接种的小鼠,其脾淋巴细胞CTL特异性释放率分别为27.34%±14.25%、32.27%±15.35%、56.28%±24.35%、44.34%±18.65%、40.76%±56%。结论BALB/c(H-2d)小鼠腹腔内接种S-HBsAg引起剂量依赖性特异性CTL反应,加强免疫能够提高CTL反应。
Objective To study whether immunization of BALB / c mice with HBV small surface vaccine (S-HBsAg) can induce specific cytotoxic T lymphocyte (CTL) responses. Methods BALB / c mice were intraperitoneally inoculated intraperitoneally with 0 ~ 6 μg S-HBsAg and once every two weeks. Splenocytes were isolated after 4 weeks and stimulated with S-HBsAg-specific CTL epitope peptide in vitro. 51Cr-labeled P815 cells were used as target cells. The 4 h51Cr release assay was used to detect CTL responses. Results The specific CTL release rates of splenic lymphocytes from mice immunized with 0, 0.65, 1.25, 2.5 and 5 μg S-HBsAg were respectively 31.21% ± 9.23%, 42.36% ± 19.32%, 91.21% ± 22.97% and 69.25% ± 24.13% and 51.49% ± 21.661%, respectively. The specific CTL release rates of spleen lymphocytes in mice immunized with only one immunization were 27.34% ± 14.25%, 32.27% ± 15.35%, 56.28% ± 24.35% and 44.34% ± 18.65%, 40.76% ± 56%. Conclusion Intraperitoneal inoculation of S-HBsAg in BALB / c (H-2d) mice induced a dose-dependent and specific CTL response, and boosting of CTL response could be enhanced by immunization.