对我国部分地区丙肝高危人群进行了较大规模的调查,证明了我国丙肝感染的严重性及丙肝抗体的应答规律;建立了HCV基因组RNA的反转录PCR方法,开展了HCV的基因检测工作。利用PCR扩增及λgt11噬菌体系统,首次构建成功我国人群HCV cDNA基因库,共获约8×10~6个噬菌斑。对此基因库进行筛选、鉴定、亚克隆及DNA测序等一系列工作,获得了我国人HCV基因组非编码区、结构区和非结构区的克隆,比较了其与国外序列的同源性和差异性,从而证实我国人群感染的属 A large-scale survey of HCV-infected population in some areas of China was carried out to prove the seriousness of hepatitis C infection and the response rules of HCV in our country. A reverse transcription PCR method of HCV genomic RNA was established to carry out genetic testing of HCV. Using PCR amplification and lambda gt11 phage system, we constructed the HCV cDNA gene library of Chinese population for the first time, obtaining about 8 × 10 ~ 6 plaques. A series of work such as screening, identification, subcloning and DNA sequencing were carried out on this gene bank. Clones of non-coding region, structural region and non-structural region of HCV genome of our country were obtained and their homologies and differences with foreign sequences were compared Sex, thus confirming the genus of infection in our population