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目的探讨过氧化物酶体增殖激活受体γ(PPARγ)激动剂罗格列酮钠(RSG)对2型糖尿病大鼠是否具有肾脏保护作用。方法 30只SD大鼠,随机分成正常对照组(n=10)、糖尿病模型组(n=10)和RSG治疗组(n=10)。糖尿病模型组和RSG治疗组予高糖高脂饮食,并结合小剂量链脲佐菌素(STZ),成功诱导2型糖尿病大鼠模型。造模成功后,RSG治疗组以RSG无菌水混悬液灌胃。4周后观察各组大鼠血清胱抑素C(CystatinC)、尿素氮(BUN)、血肌酐(Cr)、24h蛋白尿定量、血糖等生化指标并宰杀测量肾重/体质量,并加以比较。结果与糖尿病模型组相比,RSG治疗组大鼠血糖、血清胱抑素C及24h尿蛋白定量均明显降低(P均<0.05),但仍高于正常对照组(P均<0.05)。RSG治疗组肾重/体质量比值、TC及TG水平均较糖尿病模型组低(P均<0.05),与对照组比较无明显差异(P均>0.05)。3组BUN、Cr无明显变化(P>0.05)。结论 PPARγ激动剂(RSG)能明显减少2型糖尿病大鼠血糖、尿蛋白及血清胱抑素C水平,对2型糖尿病大鼠肾脏有保护作用。
Objective To investigate whether peroxisome proliferator-activated receptor γ (PPARγ) agonist rosiglitazone sodium (RSG) has renal protective effect on type 2 diabetic rats. Methods Thirty SD rats were randomly divided into normal control group (n = 10), diabetic model group (n = 10) and RSG treatment group (n = 10). Diabetic model group and RSG treatment group were given high-sugar and high-fat diet, combined with low-dose streptozotocin (STZ), successful induction of type 2 diabetic rat model. After successful modeling, the RSG treatment group was given gavage with RSG sterile water suspension. After 4 weeks, serum Cystatin C, BUN, Cr, 24h proteinuria, blood glucose and other biochemical parameters were measured and slaughtered to measure the kidney weight / body weight, and compared with each other . Results Compared with diabetic model group, the levels of blood glucose, serum cystatin C and urinary protein in 24 h in RSG treatment group were significantly decreased (all P <0.05), but still higher than those in normal control group (all P <0.05). The renal weight / body weight ratio, TC and TG levels of RSG treatment group were lower than those of diabetic model group (all P <0.05), but no significant difference compared with control group (all P> 0.05). Three groups of BUN, Cr no significant change (P> 0.05). Conclusion PPARγ agonist (RSG) can significantly reduce the level of blood glucose, urine protein and serum cystatin C in type 2 diabetic rats and protect the kidney of type 2 diabetic rats.