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目的评价单克隆抗体检测循环抗原(CAg)诊断脑囊虫病的价值。方法用猪囊尾蚴囊液抗原制备单克隆抗体4B6,以ELISA方法检测患者和对照者血清和/或脑脊液中的猪囊虫抗原。结果82例脑囊虫病患者血清CAg阳性率为79.2%(65/82),脑脊液CAg阳性率为100%(26/26),治疗一个疗程后血清CAg转阴率为85%(17/20)。从对照者标本中未检出猪囊虫CAg。4B6和包虫抗原有轻微交叉反应。结论CAg检测,尤其是脑脊液CAg检测有助于脑囊虫病的诊断,而血清CAg检测则有助于疗效考核。
Objective To evaluate the diagnostic value of monoclonal antibody for detecting cysticercosis by circulating antigen (CAg). Methods Monoclonal antibody 4B6 was prepared from Cysticercus cellulosae antigen and Cysticercus cellulosae antigens in serum and / or cerebrospinal fluid of patients and controls by ELISA. Results The positive rate of CAg in 82 patients with cerebral cysticercosis was 79.2% (65/82). The positive rate of cerebrospinal fluid CAg was 100% (26/26). After one course of treatment, the serum CAg negative rate was 85% (17/20). Cytocide CAg was not detected from the control samples. 4B6 and hydatid antigen slightly cross-reaction. Conclusion CAg test, especially cerebrospinal fluid CAg test is helpful for the diagnosis of cerebral cysticercosis, while serum CAg test is helpful to the curative effect evaluation.