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目的 探讨皮炎煎剂治疗湿疹皮炎疗效及对迟发型超敏反应作用机理。方法 用 2 ,4 -二硝氯苯 (2 ,4 -DNCB)对ICR小鼠皮肤致敏和诱发 ,制成实验性接触性皮炎模型。以中药制剂 (皮炎煎剂 1号、皮炎煎剂 2号 )作为治疗组 ,以氢化可的松琥珀酸钠为阳性对照组 ,以生理盐水为阴性对照组。对小鼠耳壳皮损严重度、耳壳增重值、耳壳皮损炎症细胞计数变化进行统计分析。结果 皮炎煎剂 1号、2号和氢化可的松对模型鼠红斑、水肿、渗出的接触性皮炎损害均有明显抑制作用 ,可减轻由于充血、水肿等炎症反应导致的局部皮损重量增加 ,减少皮损部位炎性细胞浸润。以上 3组的抗炎作用明显优于阴性对照组 (P <0 0 1)。皮炎煎剂 1号优于皮炎煎剂 2号 (P <0 0 5 )。结论 皮炎煎剂对迟发型变态反应引起的小鼠接触性皮炎具有明显抑制作用。
Objective To investigate the efficacy of dermatitis decoction in treating eczema dermatitis and the mechanism of delayed type hypersensitivity. Methods The experimental contact dermatitis model was established by sensitization and induction of skin in ICR mice with 2,4-dinitrochlorobenzene (2,4-DNCB). The traditional Chinese medicine preparations (dermatitis decoction No. 1 and dermatitis decoction No. 2) were used as the treatment group, with hydrocortisone sodium succinate as the positive control group and normal saline as the negative control group. The statistical analysis was made on the changes in the severity of auricle lesions, the increase in ear shell weight, and the number of inflammatory cells in the auricle. RESULTS: Dermatitis decoction No.1, No.2 and hydrocortisone had significant inhibitory effects on erythema, edema, and exudative contact dermatitis in model rats, which could reduce the weight of local lesions caused by inflammatory reaction such as congestion and edema. , to reduce the inflammatory cell infiltration in the skin lesions. The anti-inflammatory effect of the above three groups was significantly better than that of the negative control group (P < 0 01). Dermatitis decoction No. 1 is better than Dermatitis decoction No. 2 (P < 0 0 5 ). Conclusion Dermatitis decoction has obvious inhibitory effect on contact dermatitis caused by delayed hypersensitivity in mice.