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·AIM: To investigate micro RNA-143 expression and effect on suppression of retinoblastoma(RB) cells.· METHODS: The expression of micro RNA-143 was investigated and compared in normal human retina tissue samples and in RB cell lines of Y79 and Weri1. The micro RNA-143 mimics were transfected into the RB cell lines separately, and its effect on RB cell lines was detected using reverse-transcription quantitative polymerase chain reaction and Western blotting methods.· RESULTS: The micro RNA-143 expression was significantly suppressed in RB cell lines. Overexpression of micro RNA-143 significantly lowered cell viability and invasion of the RB cell lines, and increased the number of apoptotic cells. Meanwhile, the Bax expression was up-regulated and much higher in the micro RNA-143 mimics transfected group than that in the negative control and the micro RNA-143 inhibitor groups.· CONCLUSION: Micro RNA-143 exhibits suppressive effects in RB. The current study provides the perspective of a potential therapeutic treatment for RB.
· AIM: To investigate microRNA-143 expression and effect on suppression of retinoblastoma (RB) cells. · METHODS: The expression of micro RNA-143 was investigated and compared in normal human retina tissue samples and in RB cell lines of Y79 and Weri1 . The micro RNA-143 mimics were transfected into the RB cell lines separately, and its effect on RB cell lines was detected using reverse-transcription quantitative polymerase chain reaction and Western blotting methods. RESULTS: The micro RNA-143 expression was significantly suppressed in RB cell lines. Overexpression of micro RNA-143 significantly lowered cell viability and invasion of the RB cell lines, and increased the number of apoptotic cells. Meanwhile, the Bax expression was up-regulated and much higher in the micro RNA-143 mimics transfected group than that in the negative control and the microRNA-143 inhibitor groups. · CONCLUSION: Micro RNA-143 exhibits suppressive effects in RB. The current study provides the perspective of a potential therapeutic treatment for RB.