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牡丹皮为我国传统常用中药,安徽省铜陵地区栽培的“凤丹”根皮加工而成的药材牡丹皮被誉为道地药材,药用活性成分丰富多样,但目前尚不清楚“凤丹”药用部位次生代谢过程中活性物质合成的遗传学基础。研究采用Illumina Hi Seq 4000高通量测序平台对五年生“凤丹”根皮转录组进行测序,对测序结果进行de novo拼接和功能注释,测序后获得72 997条unigene。进一步利用公共数据库进行同源比对,其中41 139条unigene被Nr数据库成功注释,34 952条unigene能被GO数据库成功注释,20 016条unigene被KEGG数据库成功舒注释,共涉及到5个大类、34个种类、352条代谢通路;在次生物质合成与代谢途径中,其中苯丙素类化合物、萜类化合物骨架合成、各种类型萜类化合物、生物碱类化合物以及黄酮类成分生物合成途径中的unigene分别有214,104,152,55,36个;不同产地样本间差异表达基因的富集性比较显示不同产地样本间存在明显差异;此外,在72 997条unigene中共检测到9 939个SSR序列,其中二核苷酸重复的SSR标记占20.75%。研究的结果不仅为挖掘“凤丹”次生代谢物生物合成关键基因提供了基础数据信息,也为药用牡丹的遗传多样性研究和分子标记开发奠定了分子基础。
Cortex Moutan is commonly used traditional Chinese medicine in our country, Anhui Province, the cultivation of “Feng Dan ” root bark processed from medicinal material Cortex Moutan known as authentic medicine, medicinal active ingredients rich and varied, but it is not clear Feng Dan “medicinal parts secondary metabolism of the synthesis of active substances in the genetic basis. The Illumina HiSeq 4000 high-throughput sequencing platform was used to sequence the five-year-old ”Fengdan“ root bark transcriptome. The sequencing results were de novo spliced and functional annotated, and 72997 unigene were sequenced. 41 139 unigene were successfully annotated by Nr database, 34 952 unigene were successfully annotated by GO database, 20 016 unigene were successfully annotated by KEGG database, involving 5 categories , 34 species and 352 metabolic pathways. Among the pathways of secondary biomass synthesis and metabolism, phenylpropanoid, terpene skeleton synthesis, various types of terpenoids, alkaloid compounds and flavonoid biosynthesis There were 214, 104, 152, 55, and 36 unigene in the pathways respectively. The enrichment of the differentially expressed genes in different regions showed that there were significant differences among the samples from different regions. In addition, 9 939 SSR sequences were detected in 72 997 unigene, The dinucleotide repeat SSR markers accounted for 20.75%. The results not only provide the basic data for digging key genes of biosynthesis of ”Fengdan", but also lay a molecular foundation for the research on genetic diversity and molecular marker development of medicinal peony.