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创造者:西安医学院组织胚胎学教研组主任张保真教授。创造经过:过去虽有很多方法用来显示毛细胆管,但都有缺点。有的是不一定染色出来,有的染出来后仅见毛细胆管纲,看不到它和肝细胞的形态关系,有的是能够显出这种关系而操作繁琐,费时太久或者过后退色。这样给组织学实验室带来一定困难,在教学中学生往往看不到肝脏腺体结构的全貌,在研究工作中增加了时间和物力的消耗。为了解决这些问题,作者在研究肝脏组织生理的同时,根据胆酸的生物化学特性创造出一种特殊的固定剂,乙醚酒精,并结合二重包埋铁苏木素染色法,能够显示出毛细胆管来。在创造过程中,病理解剖教研组马尚德同志给予了帮助。优点:本法之优点为:
Creator: Professor Zhang Baoshen, director of Department of Embryology Teaching and Research, Xi’an Medical College. Created by: In the past though there are many ways to show the bile duct, but both have drawbacks. Some are not necessarily stained, and some dyed only see the capillary bile duct, can not see it and the morphological relationship of liver cells, and some are able to show that the relationship and cumbersome operation, take too long or after fading. This will bring some difficulties to the histology laboratory. In the teaching, students often can not see the whole picture of the liver gland structure, and increase the consumption of time and material resources in the research work. In order to solve these problems, the author in the study of liver tissue physiology at the same time, according to the biochemical characteristics of bile acids to create a special fixative, ethyl alcohol, combined with double embedding hematoxylin staining can show the bile duct . In the process of creation, Comrade Ma Shangde, a professor of pathological anatomy, gave his help. Advantages: The advantages of this law are: