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目的：研究白细胞介素－２（ＩＬ－２）脂质体对小鼠Ｂ－１６黑色素瘤肺转移瘤的抑瘤活性和对荷瘤鼠脾淋巴细胞增殖的影响。方法：改良薄膜—超声法制备ＩＬ－２脂质体；正常及免疫受抑的Ｂ－１６黑色素瘤肺转移Ｃ５７ＢＬ／６Ｎ小鼠ｉｐ游离（０．２～５×１０４ｕ／ｋｇ·ｄ×１０ｄ）或脂质体包封的ＩＬ－２（０．０４～１．０×１０４ｕ／ｋｇ·ｄ×１０ｄ），检测肺湿重、肺转移结节数和脾淋巴细胞３Ｈ－ＴｄＲ掺入量。结果：制备的ＩＬ－２脂质体为直径２００～２５００ｎｍ的大单层脂质体，包封率４８．２％。ＩＬ－２脂质体可使荷瘤鼠肺湿重下降０．４％～１３．１％，肺转移结节减少２．７～４３．５％，脾细胞３Ｈ－ＴｄＲ掺入量增加１．０５～１．９０倍，其效应相当于５倍量的游离ＩＬ－２，对免疫受抑荷瘤鼠的作用更强（Ｐ＜０．０１）。结论：ＩＬ－２脂质体对小鼠Ｂ－１６黑色素瘤肺转移瘤有显著的抑瘤活性，促进脾淋巴细胞的增殖，活性约相当于５倍量的游离ＩＬ－２。
OBJECTIVE: To study the anti-tumor activity of interleukin-2 (IL-2) liposomes on murine B-16 melanoma lung metastasis and its effect on proliferation of spleen lymphocytes in tumor-bearing mice. Methods: IL-2 liposome was prepared by modified thin film-ultrasonic method; normal and immunosuppressed B-16 melanoma lung metastasis in C57BL/6N mice was ip free (0.2～5×104u/kg·d×10d) Or liposome-encapsulated IL-2 (0.04 ~ 1.0 × 104u/kg · d × 10d), lung wet weight, pulmonary metastatic nodules and spleen lymphocyte 3H-TdR incorporation. RESULTS: The prepared IL-2 liposome was a large unilamellar liposome with a diameter of 200-2500 nm and the encapsulation efficiency was 48.2%. IL-2 liposome can reduce the weight of lungs in mice with tumors by 0.4%~13.1%, decrease the number of lung metastases by 2.7~43.5%, and increase the amount of 3H-TdR in splenocytes. From 05 to 1.90 times, the effect was equivalent to 5 times the amount of free IL-2, and the effect on immunosuppressed tumor-bearing mice was stronger (P<0.01). CONCLUSION: IL-2 liposome has significant anti-tumor activity on murine lung metastases of B-16 melanoma, and promotes the proliferation of spleen lymphocytes. The activity is equivalent to about 5 times of free IL-2.