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以八棱海棠茎尖分生组织为外植体,经农杆菌介导将PuNHA基因导入八棱海棠,在MS+BA 4 mg/L+NAA 0.2 mg/L+除草剂4 mg/L+羧苄青霉素500 mg/L的培养基中筛选培养,转化率8.7%。结果表明:经PCR、Southern Blot和Northern Blot分析得出,PuNHA基因已经整合到八棱海棠基因组内,并且可以转录为mRNA,获得了转基因植株;同时将转基因植株进行盐胁迫处理,耐盐能力有显著提高,由原来的耐盐量2‰提高到了3‰,其耐盐能力提高了50%。
In order to elucidate the effect of PuNHA gene on the growth of Malus domestica, PuNHA gene was introduced into Malus baccata with 4 mg / L NAA and 4 mg / L NAA + carbenicillin 500 mg / L medium selection and culture, the conversion rate of 8.7%. The results showed that the gene of PuNHA was integrated into the genome of B. catechu by PCR, Southern Blot and Northern Blot. The results showed that PuNHA gene was transcribed into mRNA and the transgenic plants were obtained. At the same time, transgenic plants were treated with salt stress Significantly increased from 2 ‰ salt tolerance increased to 3 ‰, its salt tolerance increased by 50%.