对As2．709碱性蛋白酶的固定化进行了研究，探讨了交联剂使用的先后程序、用量，以及载体壳聚糖与酶的比例等因素对酶固定化的影响。固定化酶最适温度为50℃，最适pH值为11，表现Km（酪蛋白）为2．4mg／ml，热稳定性良好，该酶对酿蛋白的操作半衰期在70天以上。固定化酶应用于皮革蛋白水解，45℃作用6hr，pH值从起始的10．5下降至8．5，水解度达6．4％－20．6％。应用于菜籽蛋白水解，相同水解条件下，pH值降至8．0，水解度可达13％。 The immobilization of As2.709 alkaline protease was studied. The sequence of the cross-linking agent used, the dosage, the ratio of chitosan to enzyme and other factors on the immobilization of enzyme were discussed. The optimum temperature of immobilized enzyme was 50 ℃, the optimum pH value was 11, and the Km (casein) was 2.4mg / ml. The thermostability was good. The half - life of the immobilized enzyme was above 70 days. Immobilized enzyme used in leather proteolysis, 45 ℃ for 6hr, the pH value from the beginning of 10.5 down to 8.5, the degree of hydrolysis of 6.4% -20.6%. Applied to rapeseed protein hydrolysis, the same hydrolysis conditions, the pH dropped to 8.0, the degree of hydrolysis up to 13%.