论文部分内容阅读
目的 探讨人非小细胞肺癌NCI-H1975细胞的异质性及其与药物反应性的关系.方法 利用有限稀释法获得NCI-H1975细胞单克隆化子细胞,并对母细胞和部分单克隆化子细胞行表型、药物反应性和表皮生长因子受体(EGFR)基因突变检测,分析NCI-H1975细胞的克隆异质性特征及其与药物反应性的关系.结果 获得13个单克隆化的NCI-H1975子细胞,与母细胞相比,各子细胞在细胞增殖能力、吉非替尼敏感性和顺铂敏感性方面差异有统计学意义(P＜0.05),在EGFR基因突变状态方面未见差异.结论 NCI-H1975细胞存在细胞增殖能力等表型指标和药物反应性指标的异质性,并与药物治疗的反应性和耐药相关.“,”Objective To investigate the heterogeneity of human non-small cell lung cancer cell line NCI-H1975 and its relationship with drug reactivity.Methods Monoclonal subpopulations of NCIH1975 were isolated by limiting dilution and its phenotype,drug reactivity and epidermal growth factor receptor (EGFR) gene mutation were identified.The heterogeneity of NCI-H1975 and its relationship with drug reactivity were analyzed.Results Thirteen monoclonal subpopulations of NCI-H1975 were isolated and its proliferation ability and sensitivity to gefitinib and cisplatin had significant differences compared to NCI-H1975 (P ＜ 0.05).There were no difference in EGFR gene mutation between NCI-H1975 and its subpopulations.Conclusions NCI-H1975 were heterogeneous on proliferation ability and drug reactivity and its heterogeneity were associated with sensitivity and resistance of drug treatment.