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目的:研究厚朴酚与和厚朴酚在大鼠肠道中不同部位的吸收情况及与药物浓度的关系。方法:取雄性健康Wistar大鼠麻醉后建立在体单向肠灌流模型,采用HPLC测定厚朴提取物中厚朴酚与和厚朴酚在大鼠十二指肠、空肠、回肠和结肠浓度变化,计算厚朴酚与和厚朴酚在各肠段的吸收速率常数(Ka)、表观吸收系数(Papp)和吸收百分率(AR)来研究厚朴酚与和厚朴酚的吸收动力学特征。结果:厚朴提取物中厚朴酚与和厚朴酚在大鼠十二指肠、空肠、回肠和结肠的Ka、Papp和AR差异具有统计学意义(P<0.01),均为十二指肠>空肠>回肠>结肠;不同浓度厚朴提取物中的厚朴酚与和厚朴酚在同一肠段的Ka、Papp和ARp差异无统计学意义(P>0.05)。结论:厚朴提取物中厚朴酚与和厚朴酚在大鼠肠道上段的吸收效果更好,越往肠下端吸收越差,且与提取物浓度无直接关系,其吸收机制可能为被动转移。
Objective: To study the absorption of honokiol and honokiol in different parts of the intestine of rats and its relationship with drug concentration. METHODS: Male Wistar rats were anesthetized and established in a one-way intestine perfusion model. The concentrations of magnolol and honokiol in Magnolia officinalis extract were determined by HPLC in the rat duodenum, jejunum, ileum and colon , The absorption rate constant (Ka), apparent absorption coefficient (Papp) and absorption percentage (AR) of magnolol and honokiol in each segment were calculated to study the absorption kinetics of honokiol and honokiol . Results: The difference of Ka, Papp and AR between honokiol and honokiol in rat duodenum, jejunum, ileum and colon were statistically significant (P <0.01) Intestinal> jejunum> ileum> colon; Magnolol and honokiol in different concentrations of Magnolia B had no significant difference in Ka, Papp and ARp in the same segment (P> 0.05). Conclusion: Magnolol extract and honokiol better absorption in the upper intestine of rats, the more to the lower intestine absorption worse, and the extract concentration is not directly related to the absorption mechanism may be passive Transfer.