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为了探讨下丘脑神经内分泌功能的突触调控机制和Ｐ物质（ＳＰ）神经元与γ－氨基丁酸（ＧＡＢＡ）神经元之间的相互关系，我们用包埋前免疫电镜ＰＡＰ双标技术，研究了大鼠弓状核内ＳＰ和ＧＡＢＡ神经元的超微结构分布。先以ＤＡＢ为呈色剂显示ＳＰ的免疫反应，然后用钢酸铵－ＴＭＢ法显示ＧＡＢＡ免疫反应，再经ＤＡＢ－氧化钴稳定后作免疫电镜研究。结果观察到，在弓状核内有大量含ＤＡＢ和ＴＭＢ免疫反应产物的神经元结构，ＤＡＢ反应产物为颗粒状或絮状沉淀，电子密度高，弥漫分布；ＴＭＢ反应产物呈针状或块状，散在分布。含ＤＡＢ免疫反应的结构有ＳＰ神经元的胞体、树突和轴突。含ＴＭＢ免疫反应的结构有ＧＡＢＡ神经元的胞体、树突和轴突。两种神经元均为中、小型细胞，在弓状核内混杂分布。含ＳＰ的轴突接受免疫反应阴性轴突的非对称性突触连接；含ＳＰ的树突与含ＧＡＢＡ的轴突形成对称性的轴一树突触。含ＧＡＢＡ的树突接受免疫反应用性轴突的非对称性突触连接，也接受含ＳＰ轴突的非对称性突触连接。本研究不仅在超微水平进一步证实了大鼠弓状核内存在着ＳＰ和ＧＡＢＡ神经元及其末梢，而且首次在形态学上为下丘脑氨基酸能神经元与肽能神经元之间的相互调控提供了超微结构依据。
To investigate the synaptic regulation of hypothalamic neuroendocrine function and the relationship between substance P (SP) neurons and γ-aminobutyric acid (GABA) neurons, we used immunohistochemical PAP double labeling technique Ultrastructural distribution of SP and GABA neurons in arcuate nucleus of rats. First DAB as a color reagent to show the immune response of SP, and then use ammonium acid-TMB method shows GABA immune response, and then stabilized by DAB-cobalt oxide for immunoelectron microscopy. The results showed that a large number of neurons in the arcuate nucleus containing DAB and TMB immunoreactive products, DAB reaction products were granular or flocculent precipitate, high electron density, diffuse distribution; TMB reaction products acicular or massive , Scattered distribution. Structures with DAB-immunoreaction have soma, dendrites and axons of SP neurons. TMB-immunoreactive structures include the soma, dendrites and axons of GABA neurons. Both neurons are medium and small cells, mixed in the arcuate nucleus distribution. SP-containing axons accept asymmetric synaptic connections of immunoreactive axons; SP-containing dendrites form axis-one synapses that are symmetrical with GABA-containing axons. GABA-containing dendrites accept asymmetric synaptic connections for immunoreactive axons and also accept asymmetric synaptic connections involving SP axons. This study not only further confirmed the presence of SP and GABA neurons and their terminals in the arcuate nucleus at the ultrasmall level but also for the first time morphologically the mutual regulation of amino acid neurons and peptide neurons in the hypothalamus Provides the basis of ultrastructure.