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目的：观察单价汉坦病毒灭活疫苗交叉免疫的中和抗体应答。方法：直接免疫酶斑减少中和试验、间接免疫荧光法。结果：在家兔进行单价汉坦病毒沙鼠肾细胞灭活疫苗二针基础免疫后１个月，再予另一型单价疫苗加强免疫，诱导较好的两型中和抗体应答，达到双价苗二针免疫效果；与初次免疫疫苗同型的中和抗体滴度亦显著提高。结论：汉坦病毒灭活疫苗存在交叉体液免疫反应；提示在已接受单价疫苗免疫的人群中进行交叉加强免疫，可能是有效、可靠、经济的免疫策略。
Objective: To observe the neutralizing antibody response of cross-immunization of monovalent Hantavirus inactivated vaccine. Methods: Direct immune plaque reduction neutralization test, indirect immunofluorescence method. Results: One month after the single-dose Hantaan virus gerbil kidney cell inactivated vaccine was given to rabbits, another monovalent vaccine was boosted to induce better two-type neutralizing antibody response, Miao two needle immunization effect; and primary immunization vaccine with the same type of neutralizing antibody titer was also significantly increased. Conclusion: Hantavirus inactivated vaccine cross-humoral immune response; prompt cross-booster immunization in people who have received monovalent vaccine may be effective, reliable and economical immunization strategy.