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目的 :了解河南地区人群中D16S539、D7S82 0 、D13S317基因座的遗传多态性 ,获得群体遗传数据。方法 :对2 30份无血缘关系个体的抗凝血样提取DNA ,应用多位点复合PCR扩增技术结合聚丙烯酰胺凝胶电泳分型方法调查D16S539、D7S82 0 、D13S317基因座在河南地区群体中的基因型分布。结果 :D16S539基因座在河南地区人群中存在 9个等位基因 ,2 8种基因型 ;D7S82 0 基因座存在 9个等位基因 ,2 9种基因型 ;D13S317存在 8个等位基因 ,31种基因型。基因型频率分布符合Hardy Weinberg平衡。结论 :D16S539、D7S82 0 、D13S317基因座是进行人类群体遗传学和法医遗传学研究十分有用的遗传标记。
Objective: To understand the genetic polymorphisms of D16S539, D7S82 0 and D13S317 loci in Henan population and to obtain population genetic data. Methods: DNA was extracted from anticoagulated blood samples from 230 non-related individuals. Polymorphisms of D16S539, D7S82 0 and D13S317 loci were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR) and polyacrylamide gel electrophoresis in Henan region The genotype distribution. Results There were 9 alleles and 28 genotypes in D16S539 locus in Henan province. There were 9 alleles and 29 genotypes in D7S82 0 locus. There were 8 alleles and 31 loci in D13S317 genotype. Genotype frequency distribution in line with the Hardy Weinberg equilibrium. Conclusion: The D16S539, D7S82 0 and D13S317 loci are very useful genetic markers in the research of human population genetics and forensic genetics.