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国内某些钩体病流行区已从两栖类中的蛙类分离得致病性钩体。至于蟾蜍自然感染钩体的情况,尚未见报导。我们于1977年9～11月在广州郊区对蟾蜍进行了调查,从蟾蜍肾分离得秋季热型钩体,兹报告如下。材料及方法在广州市郊新滘地区的田野和猪场附近捕捉蟾蜍。在实验室对部分蟾蜍先取血液和尿液,分别进行凝溶试验和暗视野直接镜检尿液。将蟾蜍浸泡于3%来苏液中约10～15分钟,然后剖取肾、脾脏分别培养,每种脏器接种5—氟脲嘧啶(250微克/毫升)柯氏培养基2支,置28℃,观察40天。
Some domestic leptospirosis endemic areas have been isolated from the amphibians of the frog genus Leptospira. Toad naturally infected hook body has not been reported. We conducted a survey of toads in the suburbs of Guangzhou from September to November 1977, and isolated autumn heat-type hooks from the toad kidney. We report the following. MATERIALS AND METHODS Toadstools were caught near fields and farms in the new suburb of Guangzhou. Blood and urine were taken from some toads in the laboratory, and coagulation tests and dark field were used to directly examine the urine. Toad soaked in 3% of the liquid to the Soviet Union for about 10 to 15 minutes, and then cut the kidneys, spleens were cultured, each organ inoculated 5 - fluorouracil (250 micrograms / ml) Kirschtenia 2, set 28 ℃, observed for 40 days.