AIM:To study the modulating effect of GdCl_3 and AngelicaSinensis polysaccharides (ASP) on differentially expressedgenes in liver of hepatic immunological mice by cDNAmicroarray.METHODS:Hepatic immunological injury was inducedby lipopolysaccharide (LPS ip,0.2 mg·kg~(-1)) in bacilluscalmetteguerin (BCG ip,1 mg·kg~(-1)) primed mice;A singledose of 20 mg·kg~(-1) GdCl_3 was simultaneously pretreatedand 30 mg·kg~(-1) ASP (ig,qd×7 d) was administrated whenthe BCG+LPS was primed.The mice were sacrificed atthe end of the 7~(th) day after ip LPS for 6 h and the liverwas removed quickly.The PCR products of 512 geneswere spotted onto a chemical material-coated glass platein array.The DNAs were fixed to the glass plate afterseries of treatments.The total RNAs were isolated fromthe liver tissue,and were purified to mRNAs by Oligotex.Both mRNAs from the normal liver tissue and the livertissue from the mice with hepatic immunological injury orthat pretreated with GdCl_3 or ASP were reverselytranscribed to cDNAs with the incorporation of fluorescentdUTP to prepare the hybridization probes.The mixedprobes were hybridized to the cDNA microarray.After high-stringent washing,the cDNA microarray was scanned forfluorescent signals and showed differences between thetwo tissues.RESULTS:Among the 512 target genes,18 differed in livertissue of hepatic immunological injury mice,and 6 differedin those pretreated by ASP,7 differed in those pretreatedby GdCl_3.CONCLUSION:cDNA microarray technique is effective inscreening the differentially expressed genes between twodifferent kinds of tissue.Further analysis of those obtainedgenes will be helpful to understand the molecular mechanismof hepatic immunological injury and to study the interventionof drug.Both ASP and GdCl_3 can decrease the number ofthe differentially expressed genes in liver tissue of mice withhepatic immunological injury. AIM: To study the modulating effect of GdCl_3 and Angelica sinensis polysaccharides (ASP) on differentially expressed genes in liver of hepatic immunological mice by cDNA microarray. METHODS: Hepatic immunological injury was induced by lipopolysaccharide (LPS ip, 0.2 mg · kg -1) in A singledose of 20 mg · kg -1 GdCl_3 was simultaneously pretreatedand 30 mg · kg -1 ASP (ig, qd × 7) d) was administrated whenthe BCG + LPS was primed. The mice were sacrificed atthe end of the 7 th (th) day after ip LPS for 6 h and the liver was removed quickly. The PCR products of 512 geneswere spotted onto a chemical material-coated glass platein array. The DNAs were fixed to the glass plate afterseries of treatments. The total RNAs were isolated from the liver tissue, and were purified to mRNAs by Oligotex. Both mRNAs from the normal liver tissue and the livertissue from the mice with hepatic immunological injury orthat pretreated with GdCl_3 or ASP were reverselytran scribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The mixed bacteria were hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for fluorescence signals and shows differences between the two tissues. Results: Among the 512 target genes , 18 differed in livertissue of hepatic immunological injury mice, and 6 differedin those pretreated by ASP, 7 differed in those pretreatedby GdCl_3.CONCLUSION: cDNA microarray technique is effective inscreening the differentially expressed genes between twodifferent kinds of tissue. Future analysis of these obtained genes be helpful to understand the molecular mechanism of hepatic immunological injury and to study the intervention of drug. Both ASP and GdCl_3 can decrease the number of the differentially expressed genes in liver tissue of mice with hepatic immunological injury.