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目的 研究力达霉素 (lidamycin,L DM)对内皮细胞的增殖抑制作用和诱导细胞凋亡作用。方法 用 MTT测定法和[3H]胸苷掺入测定法观察 L DM对内皮细胞的增殖抑制 ;用流式细胞分析、形态观察、蛋白质印迹分析等方法研究 L DM诱导内皮细胞凋亡及对相关调节蛋白的影响。结果 L DM呈浓度依赖性抑制内皮细胞增殖和诱导内皮细胞凋亡。L DM浓度 1～ 10 nmol/L可将内皮细胞阻断在 G2 /M期。L DM可导致内皮细胞中的游离钙增高 ,可使 Bc1-2和 PCNA蛋白的表达下调 ,但对 Bax蛋白的表达无影响。结论 力达霉素抑制内皮细胞增殖与诱导内皮细胞凋亡 ,可进一步说明其抑制血管生成的相关机制
Objective To investigate the inhibitory effect of lidamycin (L DM) on the proliferation of endothelial cells and the induction of apoptosis. Methods MTT assay and [3H] thymidine incorporation assay were used to observe the inhibitory effect of L DM on the proliferation of endothelial cells. Flow cytometry, morphological observation and Western blotting were used to investigate the effects of L DM on endothelial cell apoptosis and its correlation Regulate the effect of the protein. Results L DM inhibited the proliferation of endothelial cells and induced the apoptosis of endothelial cells in a concentration-dependent manner. L DM concentration of 1 ~ 10 nmol / L blocked endothelial cells in G2 / M phase. L DM led to the increase of free calcium in endothelial cells, down-regulated the expression of Bc1-2 and PCNA protein but had no effect on the expression of Bax protein. Conclusion Lidamycin can inhibit the proliferation of endothelial cells and induce the apoptosis of endothelial cells, which may further explain its mechanism of inhibiting angiogenesis