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通过循环伏安法(CV)将苯胺(AN)-邻氨基酚(OAP)修饰在玻碳电极(GCE)表面,制备出聚苯胺-邻氨基酚聚合物膜修饰电极(PAN-OAP/GCE),并用该电极对抗坏血酸(AA)进行测定。分别对OAP与AN聚合浓度比和磷酸缓冲溶液(PBS)pH进行优化。结果表明OAP与AN浓度比为1:14,pH为6.80时,所得聚合物膜修饰电极具有良好的电化学催化活性和稳定性。同时,在0.1 mol/L PBS(pH 6.80)中,采用差分脉冲伏安法(DPV)对AA进行测定,结果表明PAN-OAP/GCE电极对AA具有明显的电化学催化氧化作用,且AA在膜修饰电极上的响应电流和其浓度在1.50×10-8～2.12×10-6mol/L范围内呈良好的线性关系,线性回归方程为ip=1.0344c+0.0183,相关系数为0.9988。检测限可达5.0×10-9mol/L。该修饰电极具有较高的灵敏度和选择性,用于样品中AA的检测,回收率为96.0%～101.2%。
The polyaniline-o-aminophenol polymer membrane modified electrode (PAN-OAP / GCE) was prepared by cyclic voltammetry (CV) on the surface of glassy carbon electrode (GCE) , And ascorbic acid (AA) was measured using this electrode. The concentrations of OAP and AN polymerization and the pH of phosphate buffered saline (PBS) were optimized respectively. The results showed that the ratio of OAP to AN was 1: 14 and the pH was 6.80. The obtained polymer film modified electrode had good electrochemical catalytic activity and stability. At the same time, AA was determined by differential pulse voltammetry (DPV) in 0.1 mol / L PBS (pH 6.80). The results showed that PAN-OAP / GCE had obvious electrochemical catalytic oxidation effect on AA, The response current and the concentration of the modified electrode showed good linearity in the range of 1.50 × 10-8 ~ 2.12 × 10-6mol / L, the linear regression equation was ip = 1.0344c + 0.0183, the correlation coefficient was 0.9988. Detection limit of up to 5.0 × 10-9mol / L. The modified electrode with high sensitivity and selectivity for the detection of AA in the sample, the recovery was 96.0% ~ 101.2%.