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目的 :观察高原低氧大鼠下丘脑谷氨酸 (Glu)、天门冬氨酸 (Asp)和一氧化氮合酶 (NOS)的变化。方法 :应用氨基酸测定和NADPH -d组化法 ,检测高原低氧模型大鼠下丘脑Glu、Asp含量和NADPH -d阳性神经元的数量。结果 :高原低氧大鼠下丘脑Glu、Asp含量明显增多 ,室旁核、视上核可见密集深染的NADPH -d阳性神经元 ;用NMDA受体拮抗剂氯氨酮 (Ketamine)和AP -V对高原低氧大鼠进行预处理后置于低压氧舱 ,观察到大鼠下丘脑室旁核、视上核NADPH -d阳性神经元数明显少于相应时间的高原低氧组 (P <0 0 1)。结论 :NMDA受体可能参与了高原低氧引起的下丘脑NOS的表达。
Objective: To observe the changes of glutamate (Glu), aspartate (Asp) and nitric oxide synthase (NOS) in hypothalamus of plateau hypoxia rats. Methods: Amino acid assay and NADPH-d histochemical method were used to detect the contents of Glu and Asp in the hypothalamus and the number of NADPH-d positive neurons in the plateau hypoxia model rats. Results: The contents of Glu and Asp in the hypothalamus were significantly increased in the plateau hypoxia rats. NADPH-d positive neurons in the paraventricular nucleus and supraoptic nucleus were densely stained. Using the Ketamine and AP - V preconditioned on plateau hypoxic rats were placed in the hyperbaric oxygen chamber, observed in rat hypothalamic paraventricular nucleus, supraoptic nucleus NADPH-d positive neurons were significantly less than the corresponding plateau hypoxia group (P < 0 0 1). Conclusion: NMDA receptor may be involved in hypothalamic NOS expression induced by plateau hypoxia.