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目的探讨载脂蛋白B基因(apolipoproteinB,apoB)G11039A多态与脑血管病的关系。方法采用聚合酶链式反应-限制性片段长度多态性分析法(PCR-RFLP)分析130例脑梗死患者、130例脑出血患者、100例对照组人群apoB基因G11039A多态;并采用氧化酶法测定血清甘油三脂(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL),运用酶联免疫吸附法测定LP(a)浓度,免疫比浊法测定apoB100及apoAI浓度。结果脑梗死组apoB基因G11039A多态A等位基因频率(0.065)高于对照组(0.015)(P<0.05);脑出血组apoB基因G11039A多态A等位基因频率(0.023)与对照组无显著差别。脑梗死组及脑出血组中,G/G或G/A基因型亚组的各项血脂水平无显著差别(P>0.05)。结论apoB基因G11039A多态可能与脑梗死有关,而与脑出血无关。
Objective To investigate the relationship between apolipoprotein B (apoB) G11039A polymorphism and cerebrovascular disease. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the polymorphism of apoB gene G11039A in 130 patients with cerebral infarction, 130 patients with cerebral hemorrhage and 100 controls. The oxidative stress (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) were determined by enzyme linked immunosorbent assay (ELISA) apoB100 and apoAI concentrations. Results The frequency of allele A110 of G11039A (0.065) in apoB gene group was significantly higher than that in control group (P <0.05) in cerebral infarction group. The allele frequency of allele A110 of ApoB gene (0.023) Significant difference. There were no significant differences in serum lipids between subgroups of G / G or G / A genotypes in cerebral infarction group and intracerebral hemorrhage group (P> 0.05). Conclusion ApoB gene G11039A polymorphism may be related to cerebral infarction, but not to cerebral hemorrhage.