论文部分内容阅读
【目的】通过调查广东省矿泉水和山泉水生产企业水源水、碳后水和成品水中的粪链球菌(Enterococcus faecalis)污染情况,为生产企业微生物控制提供相应的依据。【方法】粪链球菌的检测方法采用稍作修改的GB/T8538-2008/4.53,并运用ERIC-PCR技术对主要污染菌株进行分型。【结果】206份水样中有35份水样检出粪链球菌,其中水源水20份、碳后水13份和成品水2份,水源水、碳后水和成品水的污染率分别为26.3%、20%和3.1%,总污染率为17%。矿泉水和山泉水的总污染率分别为3.8%和25.2%,山泉水、地下水和地表水的水源污染率分别为33.3%和63.6%。ERIC-PCR指纹图谱聚类分析显示35株菌分为3簇,主要污染菌基因型在B簇。【结论】广东省山泉水的粪链球菌污染率明显高于矿泉水的污染率,同时山泉水的水源水污染率中,地表水高于地下水。
【Objective】 The objective of this study was to investigate the contamination of Enterococcus faecalis in tap water, post-carbonated water and finished product water of mineral water producers and spring producers in Guangdong Province. 【Method】 The detection method of Streptococcus faecalis was modified slightly GB / T8538-2008 / 4.53, and the major contaminating strains were classified by ERIC-PCR. 【Result】 Twenty-five samples of 206 water samples were collected for detection of Streptococcus faecalis. Among them, 20 were water source water, 13 carbon after water and 2 finished product water. The pollution rates of water source water, carbon water and finished water were 26.3%, 20% and 3.1%, the total pollution rate was 17%. The total pollution rates of mineral water and spring water were 3.8% and 25.2% respectively, and the water pollution rates of spring water, groundwater and surface water were 33.3% and 63.6% respectively. The cluster analysis of ERIC-PCR fingerprinting showed that 35 strains were divided into three clusters, and the major pollutant genotypes were in cluster B. 【Conclusion】 The contamination rate of streptococcus faecalis in mountain spring water of Guangdong Province is obviously higher than that of mineral water, and the surface water of surface spring water is higher than that of groundwater in spring water of mountain spring.