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应用冰冻旋毛虫幼虫微量沉淀试验检测感染旋毛虫的大鼠、兔及患者血清。结果：抗体阳性率分别为１００％，１００％及９１．６７％，而正常大鼠、兔、健康人及其它寄生虫病患者血清除１例囊虫病患者外，均为阴性；用新鲜幼虫试验沉淀反应常见于口孔或肛孔处，而用冰冻幼虫时，其反应可见于幼虫整个体表。结果表明，冰冻幼虫微量沉淀试验具有高度的敏感性和特异性，且抗原制备简单并可长期使用，对旋毛虫病的免疫诊断有实用价值。
Serum of rats, rabbits and patients infected with Trichinella spiralis were detected by microtiter sedimentation assay of frozen Trichinella spiralis larvae. Results: The positive rates of antibody were 100%, 100% and 91.67%, respectively. However, the serums of normal rats, rabbits, healthy people and other parasitic patients were all negative except one case of cysticercosis. Experimental precipitation reaction is common in the mouth or anal hole, and with frozen larvae, the reaction can be seen in the larval body surface. The results showed that the frozen larvae microprecipitation assay is highly sensitive and specific, and the antigen preparation is simple and can be used for a long time, and has practical value for immune diagnosis of trichinosis.