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以原核表达并纯化人胸腺素α原后，观察其体外活性，了解到胸腺素α原对胸腺细胞氢化考的松损伤有明显的保护作用，表现在它可明显提高氢化考的松损伤后细胞的数目和ＭＴＴ反应能力，氢化考的松引起胸腺细胞凋亡的发生，并显著降低ＣＤ４＋、ＣＤ８＋单阳性细胞和ＣＤ４－ＣＤ８－双阴性细胞的比例，胸腺素α原不能抑制胸腺细胞凋亡的发生，并可能对胸腺细胞凋亡有促进作用，与空白对照组比较，它也可明显降低ＣＤ４＋阳性细胞的比例，结果表明，胸腺素α原对胸腺细胞氢化考的松损伤的保护作用并非通过抑制凋亡而实现。
After prokaryotic expression and purification of human thymosin α, the activity in vitro was observed. It was found that thymosin α had a significant protective effect on the hydrogenation of thromboxane-induced thrombosis in thymocytes, And the ability of MTT reaction, cortisol induced apoptosis of thymus cells, and significantly reduced the proportion of CD4 +, CD8 + single positive cells and CD4-CD8- double negative cells, thymosin alpha can not inhibit thymocyte apoptosis And may promote the apoptosis of thymus cells. Compared with the blank control group, it can also significantly reduce the proportion of CD4 + positive cells. The results showed that the protective effect of thymosin α on thrombus hydrogenation of cortisone is not through Inhibition of apoptosis and achieve.