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目的了解目前甘肃结核分枝杆菌流行株的基因型特征,并评价两种基因分型方法的应用价值。方法收集甘肃省结核分枝杆菌临床分离菌株,分别采用多位点数目可变串联重复序列分析(multiple-locus variable number tandem repeat analysis,MLVA)和间隔区寡核苷酸分型(spacer oligonucleotide typing,Spoligotyping)技术进行基因分型,用BioNumerics软件进行聚类分析后,进行结果综合分析、比对。结果利用MLVA分型方法,228株结核分枝杆菌被分为4大基因群,7个主要基因型;Spoligotyping可将228株结核分枝杆菌分为4个基因群,8个主要基因型,其中最大的基因型为北京家族基因型。两种方法在基因型分型方面经卡方检验差异无统计学意义(χ2=0.06,P>0.05)。结论两种方法对结核分枝杆菌的分型效果良好,重复性高,操作简便,可试用于大规模分子流行病学调查。北京家族是甘肃最主要的流行基因群。
Objective To understand the genotypes of Mycobacterium tuberculosis strains currently prevailing in Gansu and to evaluate the application value of the two genotyping methods. Methods The clinical isolates of Mycobacterium tuberculosis in Gansu province were collected and analyzed by multiple-locus variable number tandem repeat analysis (MLVA) and spacer oligonucleotide typing Spoligotyping) technology for genotyping, using BioNumerics software cluster analysis, the results of a comprehensive analysis, comparison. Results 228 strains of Mycobacterium tuberculosis were divided into 4 major gene groups and 7 major genotypes by MLVA typing. Spoligotyping divided 228 strains of Mycobacterium tuberculosis into 4 gene groups and 8 major genotypes The largest genotype is Beijing family genotype. The two methods in the genotyping by chi-square test showed no significant difference (χ2 = 0.06, P> 0.05). Conclusion The two methods have good typing effect on Mycobacterium tuberculosis, high repeatability, simple and convenient operation, and can be used for large-scale molecular epidemiological investigation. Beijing family is the most popular genotype Gansu.