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本研究选用了32个栽培高粱品种、10个苏丹草品种及2个高粱近缘种进行了RAPD分析。结果表明,1)12对引物产生的68条DNA扩增片段中,52条(76.5%)具有多态性。2)高粱之间的相似系数从55%到95%;苏丹草之间的相似系数从52%到84%,因此选择的品种之间的多态性高,具有代表意义。高粱不育系和保持系之间的相似度很大,为89%以上,但通过RAPD标记能够将其区分。3)以0.66为阈值将44个品种分为10个类群。第1类群中全部为苏丹草;在2,3,4群中既有苏丹草也有高粱;5,6,7,8群中则全部为高粱。因此,使用RAPD分子标记进行聚类不能将高粱和苏丹草区分开来。
In this study, 32 cultivated sorghum varieties, 10 sudangrass varieties and 2 sorghum related species were selected for RAPD analysis. The results showed that 1) of the 68 DNA fragments amplified by 12 pairs of primers, 52 (76.5%) were polymorphic. 2) The similarity coefficient between sorghums ranges from 55% to 95%, while the similarity coefficient between Sudan grasses ranges from 52% to 84%. Therefore, the polymorphism among the selected varieties is highly representative. The similarity between sorghum CMS lines and maintainer lines is very high at 89%, but they can be distinguished by RAPD markers. 3) Divided 44 cultivars into 10 groups with 0.66 as the threshold. The first group of all sudangrass; in groups 2, 3, 4 both sudangrass and sorghum; 5,6,7,8 group are all sorghum. Therefore, clustering using RAPD markers can not distinguish sorghum from sudangrass.