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目的研究反复自然流产(RSA)患者妊娠绒毛组织中细胞凋亡抑制蛋白survivin与促凋亡分子Bax的表达情况,分析绒毛滋养层细胞凋亡与RSA的关系。方法用免疫组化染色法检测妊娠12周以内RSA与同期正常人流组绒毛滋养层细胞中survivin、Bax的表达,用TUNEL法检测2组绒毛组织滋养层细胞中的细胞凋亡。结果 RSA组绒毛滋养层细胞中survivin表达明显低于正常人流组(P<0.05),Bax表达明显高于正常人流组(P<0.05),RSA组绒毛滋养层细胞凋亡指数(AI)明显高于正常人流组(P<0.05),RSA组中survivin与Bax的表达呈负相关(r=-0.51,P<0.05)。结论在RSA患者绒毛滋养层细胞中,survivin表达下降、Bax表达上升。
Objective To study the expression of survivin and Bax in gestational chorion in patients with recurrent spontaneous abortion (RSA), and to analyze the relationship between apoptosis of chorionic villus and RSA. Methods The expressions of survivin and Bax in trophoblast cells of RSA group and normal human flow group were detected by immunohistochemical staining in 12 weeks gestation. The apoptosis of trophoblast cells in 2 groups was detected by TUNEL method. Results The expression of survivin in the trophoblast cells of RSA group was significantly lower than that in normal human flow group (P <0.05), and the expression of Bax in RSA group was significantly higher than that in normal human flow group (P <0.05). The apoptosis index (AI) In normal human flow group (P <0.05), the expression of survivin and Bax in RSA group was negatively correlated (r = -0.51, P <0.05). Conclusion In the trophoblast cells of RSA patients, the expression of survivin decreased and the expression of Bax increased.