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目的 在CVB3 易感的Vero细胞系中观察与CVB3RNA 5’NCR的nt 5 81- 6 0 1区域互补的 2 1聚硫代AODN抗病毒活性。方法 采用MTT法测定细胞活性 ,直接观察CPE ,测定培养上清TCID50 ,并分别用ELISA和斑点杂交法检测CVB3 抗原及其RNA。结果 1.AODN可推迟和减轻CPE ,且随AODN浓度增加 ,对CPE的抑制作用增强 ,感染细胞存活率也随AODN浓度升高而升高 ;2 .AODN可抑制CVB3 抗原表达及RNA的合成 ,且呈剂量依赖关系 ;3.培养上清中病毒滴度随AODN浓度升高而降低。AODN抗病毒活性在转染后 48h效果较好 ;10 μmol/L浓度抑制效果较好。本研究结果也显示 10 μmol/LSODN对CVB3 感染细胞CPE有较弱的抑制作用 ,但RODN未显示抗病毒活性 ;AODN未显现对HSV - 1感染细胞CPE抑制活性 ,但对其他肠道病毒如CVB5,Polio - 1和ECHO - 6有较弱的抑制活性。结论 AODN可能通过抑制CVB3RNA复制来抑制病毒合成
OBJECTIVE To observe the antiviral activity of 2 1 polythio-AODN complementary to the nt 5 81-6 0 1 region of the 5 ’NCR of CVB3 RNA in a Vero cell line susceptible to CVB3. Methods The cell viability was measured by MTT method. CPE was directly observed and the TCID50 of culture supernatant was measured. The CVB3 antigen and RNA were detected by ELISA and dot blot hybridization. AODN can delay and reduce CPE, and with the increase of AODN concentration, the inhibitory effect on CPE is enhanced, and the survival rate of infected cells also increases with the increase of AODN concentration; 2.AODN can inhibit the expression of CVB3 antigen and RNA synthesis, And in a dose-dependent manner.3. The virus titer in the culture supernatant decreased with the increase of AODN concentration. The antiviral activity of AODN was better at 48h after transfection; the inhibitory effect at 10 μmol / L was better. The results of this study also showed that 10 μmol / L ODN had a weaker inhibitory effect on CPE of CVB3 infected cells, but RODN did not show antiviral activity. AODN did not show CPE inhibitory activity on HSV - 1 infected cells, but other enteroviruses such as CVB5 Polio - 1 and ECHO - 6 have weaker inhibitory activity. Conclusion AODN may inhibit the virus synthesis by inhibiting the replication of CVB3 RNA