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缺氧微环境是实体肿瘤的特征之一,也是影响实体肿瘤发生、发展、侵袭、转移及耐药的重要因素,但其影响肿瘤的具体机制尚未完全明确。根据检测的mRNA数量,基因芯片分为传统的表达谱芯片和功能分类芯片。随着技术的发展,基因芯片技术因能对成千上万种基因的表达情况同时进行定量检测,在生命科学研究包括缺氧微环境对肿瘤作用的研究中越来越多地得到应用。选择性应用这些基因芯片除了能证明缺氧微环境与肿瘤发生发展有关外,同时还能筛选肿瘤缺氧微环境调节的未知靶基因及缺氧微环境重要调节因子的未知下游靶基因,还能为研究缺氧微环境影响肿瘤的分子机制提供有用的线索。基因芯片技术正成为该领域非常重要的研究工具之一。
Hypoxic microenvironment is one of the characteristics of solid tumors, but also an important factor affecting the occurrence, development, invasion, metastasis and drug resistance of solid tumors. However, the exact mechanism of tumorigenesis is not yet fully understood. According to the number of mRNA detected, the gene chip is divided into traditional expression chip and functional classification chip. With the development of technology, gene chip technology can quantitatively detect the expression of thousands of genes at the same time. It has been applied more and more in the research of life science including hypoxia microenvironment on tumor function. Selective application of these gene chips in addition to demonstrating hypoxia microenvironment and tumor development related to the same time, screening of unknown hypoxia micro-environment regulation of unknown target genes and hypoxia microenvironment important regulators of unknown downstream target genes, but also It provides useful clues for studying the molecular mechanism of hypoxia and microenvironment affecting tumor. Gene chip technology is becoming one of the most important research tools in this field.