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目的建立一种新的模拟深低温停循环条件下肺缺血再灌注大鼠模型。方法 Wister大鼠30只,分为实验组、对照组和正常组。麻醉后气管切开插管接小动物呼吸机,股动脉置管测动脉压力,监测心电、肛温、血氧饱和度和血气分析,用体外降温至肛温24℃。实验组阻断左下肺门30min后开放,并逐渐将体温恢复正常;对照组单纯降温复温。复温后处死动物,取肺组织标本计算W/D值及病检。结果实验大鼠无死亡。实验组W/D值较正常组和对照组明显增高(P<0.05)病检显示实验组明显肺泡间质水肿,大量炎性细胞、红细胞渗出;而对照组病变明显减轻。结论 本实验方法制作的大鼠模型可以用于模拟人深低温停循环肺缺血再灌注的研究。
Objective To establish a new model of lung ischemia-reperfusion injury in simulated hypothermic circulatory arrest. Methods Thirty Wister rats were divided into experimental group, control group and normal group. After anesthesia tracheotomy intubation small animal ventilator, femoral artery catheter arterial pressure, monitoring ECG, rectal temperature, oxygen saturation and blood gas analysis, with an external temperature to rectal temperature 24 ℃. The experimental group blocked the left lower hilar 30min after opening, and gradually returned to normal body temperature; the control group simply cooling and rewarming. After rewarming sacrificed animals, lung tissue samples were taken to calculate the W / D value and disease detection. Results There was no death in experimental rats. The W / D value in the experimental group was significantly higher than that in the normal group and the control group (P <0.05). The pathological examination showed obvious alveolar interstitial edema, a large number of inflammatory cells and erythrocyte exudation in the experimental group, while the lesion in the control group was significantly reduced. Conclusion The rat model made in this experiment can be used to simulate the hypothermic circulatory ischemia-reperfusion injury in human.